Product Description
ONLY FOR SCIENCE RESEARCH
Drug Names
Generic Name：Human anti-2019 nCoV(S) IgMELISA Kit(Qualitative)
Principle
This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombinant 2019 nCoV spike protein (antigen) was pre-coated onto 96-well plates. The test samples were added to the wells, unbound conjugates were washed away with wash buffer. Then added HRP conjugated anti-human IgM, if there were any anti-2019-nCoV(S)-IgM in the samples, it would form a complex. TMB substrates were used to visualize HRP enzymatic reaction. It was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650nm.
Materials provided with the kit
|
Item |
Specifications(96T) |
Storage |
|
ELISA Microplate(Dismountable) |
8×12 |
2-8°C |
|
Negative Control (Ready-to-use) |
1vial |
2-8°C |
|
Positive Control (Ready-to-use) |
1vial |
2-8°C |
|
Sample Dilution Buffer |
1vial |
2-8°C |
|
HRP-conjugated anti-human IgM antibody (Concentrated) |
1vial |
2-8°C |
|
Antibody Dilution Buffer |
1vial |
2-8°C |
|
Wash Buffer (25 x concentrate) |
1vial |
2-8°C |
|
TMB Substrate |
1vial |
2-8°C |
|
Stop solution |
1vial |
2-8°C |
|
Plate Sealer |
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